Breakthrough new stereomicroscope optics deliver the highest zoom range possible, while maintaining high resolution and exceptionally bright, uniform fluorescence
Nikon’s SMZ25 stereomicroscope combines macro and micro imaging in one instrument for convenient viewing and manipulation of single cells to whole organisms. Using Nikon’s Perfect Zoom System optical technology, the SMZ25 achieves the world’s first 25:1 zoom and high NA for superior resolution never before seen with a stereomicroscope.
Using these innovative optics and a fly-eye lens, crystal clear fluorescent images with improved S/N ratios are possible, even in low-excitation light applications such as cell division. Additionally, even at low magnifications, the SMZ25 has a 35mm F.O.V, allowing users to capture an entire 35mm dish with uniform brightness.
"Perfect Zoom System" Offers New Levels of Imaging Power and Versatility
A breakthrough in stereomicroscope design, the SMZ25’s Perfect Zoom System dynamically changes the distance between the two optical axes as the zoom factor varies. This change in optical axis distance enables maximization of light entry into the optical system at every magnification. The result is a large zoom range, high resolution in both eye-paths, and minimal aberrations over the entire zoom range.
World's Largest Zoom Range Enables High Resolution Macro to Micro Imaging
Nikon’s exclusive Perfect Zoom System provides the world’s first zoom ratio of 25:1 (zoom range: 0.63x - 15.75x*). Even with a 1x objective lens, the SMZ25 captures the entire 35mm dish and simultaneously delivers microscopic details.
Auto Link Zoom (ALZ) Provides Seamless Viewing at Different Magnifications
The SMZ25’s exclusive Auto Link Zoom (ALZ) automatically adjusts the zoom factor to maintain the same field of view when switching objective lenses.
This function enables seamless switching between whole organism imaging at low magnifications and detailed imaging at high magnifications.
Brighter and Higher Contrast Epi-fluorescence Imaging
Even at low magnification, the SMZ25 captures an entire 35mm dish with equal brightness over the whole field of view, making it ideal for live screening of developmental models such as C.elegans,Drosophila, zebrafish, and mice to identify and select mutants.
The SMZ25 also allows brilliant images to be captured even with low excitation light levels, minimizing photo-bleaching and photo-toxicity which is harmful to live cells and organisms.
The SMZ25 combines fly-eye technology, which uniformly illuminates the entire field of view, and a high S/N ratio along with increased transmission for incredibly bright fluorescence, even at low magnifications. This also minimizes photobleaching and phototoxicity, thus reducing harm to live cells and organisms.
Nikon’s newly developed optical system offers a drastic improvement in S/N ratio even at high magnifications.
The improved S/N ratio makes it possible to capture cell division, which is difficult using conventional stereomicroscopes, and samples with low excitation light.
Advanced Motorization and Digital Imaging Capabilities
On-axis Imaging for Digital Images
Easily switch between stereo position (stereoscopic view) and mono position (on-axis view) when using the P2-RNI2 Intelligent Nosepiece by simply sliding the objective lens. Digital images with uncompromised clarity can be captured using the mono position.
The all-new controller provides easy access to zoom and focus controls and is designed for both right- and left-hand use. The remote controller contains an LCD monitor with an adjustable backlight which provides information regarding the zoom factor, objective lens, filter cube, and LED DIA brightness at a glance. The backlight on the LCD monitor can also be turned off to eliminate interference with low-light imaging applications. In addition to the remote controller, the microscope can also be operated through a PC.
NIS-Elements Imaging Software
Nikon’s flagship, cross-platform imaging, software can now be used with Nikon’s newest stereomicroscope systems. NIS-Elements enables a wide range of advanced digital imaging capabilities, easily from a PC.
Digital Sight DS-L3 Digital Camera System
The DS-L3 is an easy-to-use high-definition, large touch-panel monitor that can be used to quickly capture images without a PC.
Wide Range of Available Accessories
Base Unit Options
Nikon has improved ease of use by moving the controls to the front of the base, including the brightness adjustment dial and on/off switch.
- Fiber DIABase - Fiber DIA base features condenser lenses that can be switched between low and high magnifications. Furthermore, the Oblique Coherent Contrast (OCC) illumination system allows high contrast illumination.
- Slim Bases - The slimmer LED DIA Base and Plain Base help increase efficiency of sample manipulation by bringing the level of the sample closer to the table.
The focus unit is combined with the base unit. Choose from either a manual or motorized focus unit.
SHR Plan Apo Series Objective Lenses
The SHR Plan Apo series features higher NA, wider field of view, and superior flatness and color aberration correction. These objective lenses can be seamlessly switched because all magnifications have the same parfocal distance. The new bayonet mount design allows lenses to be safely and easily removed.
Choose from two types of tilting trinocular tube and one type of low eyelevel trinocular tube. All tubes have a camera port for seamless integration with the Digital Sight series.
Nosepiece / Focus Mount Adapter
There is one type of nosepiece that can mount two objective lenses and two types of focus mount adapters for use with a single objective lens.
The stage features an XY stroke of 6x4 inches (150mm x 100mm) and can be attached to any of the bases, making it effective for capturing large images when used in combination with the imaging software NIS-Elements. A sliding stage and tilting stage are also available.
Nikon offers a remote control unit that can be used to operate the microscope and capture images by hand. A footswitch is also offered, allowing the user to operate the microscope and capture images by foot, freeing the hands for sample manipulation.
Epi-fluorescence Light Sets
- Motorized: The fluorescent turret can be operated using the remote control or imaging software NIS-Elements.
- Manual: An easy-to-use manual model for Nikon’s newly developed high performance epic-fluorescence atta.
Fiber Illuminator Sets
- Flexible Double Arm Fiber Illumination Set: The direction and angle of illumination can be changed to suit the sample by making adjustments with these double arms. The fiber holder position can also be changed to obtain the optimal position for illuminating samples.
- Manual Epi-Fluorescence Light Set: This ring fiber illumination set features an episcopic illumination unit that effectively captures images (can be used with 1x and 0.5x objective lenses).
The coaxial light illuminator makes it possible to view light reflected from the surface of a sample, which is ideal for shooting shadow-less images of thick samples.
Ring LED Illuminator
This ring fiber illumination set features an episcopic illumination unit that effectively captures images (can be used with 1x and 0.5x objective lenses).
Darkfield Observation Accessory
Darkfield viewing is possible simply by attaching the darkfield unit to the base.
Polarizing Observation Accessory
The analyzer is attached to the objective and the polarizer to the base or stand to enable polarized viewing.
Apochromatic optical system
Zooming body built-in
|Objectives NA, WD (mm)|
|P2-SHR Plan Apo||
2x 0.312, 20
(with a correction ring for water 0 to 3mm in depth)
|P2-SHR Plan Apo||
1.6× 0.25, 30
|P2-SHR Plan Apo||
1× 0.156, 60
|P2-SHR Plan Apo||
0.5× 0.078, 71
|Total Magnification (Using 10x eyepieces)||
(Depending on objective used)
|Eyepieces (F.O.V. mm)||
C-W 10xB(22) , C-W 15x(16) , C-W 20x(12.5) , C-W 30x(7)
P2-TERG 100 Trinocular Tilting tube (100/0 : 0/100), P2-TERG 50 Trinocular Tilting tube (100/0 : 50/50) Inclination angle : 0°~30°, P2-TL100 Trinocular Tube L (100/0 : 0/100) Inclination angle : 15°
|Focus Unit (Stroke from Objective's parfocal point)||
P2-MFU Motorized Focus Unit (Up 96mm/Down 4mm)
|Focus Mount Adapter/Nosepiece||
P2-FM Focus Mount Adapter, P2-RNI2 Intelligent Nosepiece
(2 objectives can be attached)
P2-PB Plain Base , P2-DBL LED Diascopic Illumination Base (OCC illuminator built-in), P2-DBF Fiber Diascopic Illumination Base , P-PS32 Plain Stand (Only for SMZ18)
P-SXY64 Stage , C-SSL Dia-sliding Stage , C-TRS Tilting Stage
4 filter cubes mountable, Fly eye lens built-in, P2-EFLM Motorized Epi Fluorescence Attachment.
|Epi-Fluorescence Light Sources||
HG Precentered Fiber illuminator Intensilight C-HGFIE HG/C-HGFI HG (130W)
P2-FIRL LED Ring Illumination Unit
Use with Fiber light source, P2-CI Coaxial Epi Illuminator , P2-FIR Ring Fiber Illumination Unit, C-FDF Flexible Double Arm Fiber Illumination Unit
|Episcopic Light Sources||
C-FLED2 LED Light source for fiber illuminator
(Episcopic) Coaxial Epi Illuminator, Epi Fluorescence Illuminator, Ring LED Illuminator
(Motorized Epi Fluorescence Attachment configuration with Trinocular Tilting Tube, Motorized Focus Unit, Intelligent Nosepiece, LED DIA base and Objectives 1x and 0.5x)
|Power Consumption (approx.)||
(Motorized Epi Fluorescence Attachment configuration with Trinocular Tilting Tube, Motorized Focus Unit, Intelligent Nosepiece and LED DIA base)
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